|
|
|
|
|
| Gioria,Ricardo; Braga,Renato de Souza; Krause-Sakate,Renate; Roullier,Caroline; Rosa,Daniel Dias; Moura,Monika Fecury; Souza-Dias,José Alberto Caram de; Sawazaki,Haiko Enok; Camargo,Luis Eduardo Aranha; Rezende,Jorge Alberto Marques. |
| Plants of Capsicum annuum cv. Magali R, resistant to Pepper yellow mosaic virus (PepYMV), which showed severe yellow mosaic, leaf malformation and stunting were observed during the 2003/04 growing season in Lins, São Paulo State, Brazil. Potyvirus-like particles observed in leaf sap from infected plants under the electron microscope reacted with an antiserum against PepYMV in PTA-ELISA. In addition to C. annuum cv. Magali R, this potyvirus also infected systemically the resistant C. annuum cv. Rubia R. The nucleotide sequence of part of the CP gene of this potyvirus shared 96-98% identity with that of other PepYMV isolates. The partial nucleotide sequence of the 3' NTR showed 94-96% identity with that of PepYMV. These data indicate that this potyvirus is a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Potyvirus; RT-PCR; ELISA; Resistance gene. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000200017 |
| |
|
|
| Maciel,Sheila Conceição; Silva,Ricardo Ferreira da; Reis,Marcelo Silva; Jadão,Adriana Salomão; Rosa,Daniel Dias; Giampan,José Segundo; Kitajima,Elliot Watanabe; Rezende,Jorge Alberto Marques; Camargo,Luis Eduardo. |
| Catharanthus roseus is a perennial, evergreen herb in the family Apocynaceae, which is used as ornamental and for popular medicine to treat a wide assortment of human diseases. This paper describes a new potyvirus found causing mosaic symptom, foliar malformation and flower variegation in C. roseus. Of 28 test-plants inoculated mechanically with this potyvirus, only C. roseus and Nicotiana benthamiana developed systemic mosaic, whereas Chenopodium amaranticolor and C. quinoa exhibited chlorotic local lesions. The virus was transmitted by Aphis gossypii and Myzus nicotianae. When the nucleotide sequence of the CP gene (768nt) was compared with other members of the Potyviridae family, the highest identities varied from 67 to 76 %. For the 3' UTR (286nt),... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: RT-PCR; Apocynaceae; Periwinkle; Diagnose; Genome sequencing. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000600013 |
| |
|
| |
|
|
|
